The following data were obtained from partial cleavage and analysis of a pentadecapeptide:?

Question

The following data were obtained from partial cleavage and analysis of a pentadecapeptide:CNBr digestion peptides (not in order): CNBr1: Ala, Gly, Leu, Lys, Met, Pro CNBr2: Ala, Arg, Asp, Cys, Gln, Gly CNBr3: Met, Phe, Trp Trypsin digestion peptides (not in order): T1: Ala, Cys, Gln T2: Ala, Gly, Lys, Met, Phe, Trp T3: Arg, Asp, Gly, Leu, Met, Pro N-terminus: Phe (for the pentadecapeptide) Gly (for CNBr1) Asp (for CNBr2) Cys (for T1) Leu (for T3) C-terminus: GlnDetermine the sequence of the pentadecapeptide. Use three-letter abbreviations in your answer. Use dashes (–) between each abbreviation.

Major Spinka DVM · Accepted Answer

Phe- Trp- Met- Gly- Ala- Lys- Leu- Pro- Met- Asp- Gly- Arg- Cys- Ala- GlnCyanogen bromide cleaves after Met residues; trypsin cleaves after Lys and Arg residues. From the given data, the residues are Ala (2), Gly (2), Met (2), Leu (1), Lys (1), Pro (1), Arg (1), Asp (1), Cys (1), Gln (1), Phe (1), Trp (1). From the data, you can write the first and last residues of the pentadecapeptide. The first residue (N-terminus) is Phe; the last residue (C-terminus) is Gln. You are also given the first and last residues for the CNBr1, CNBr2, T1, and T3 peptides. T1: the first residue is Cys (given). There are no Lys or Arg residues, so this must be at the C-terminus of the pentadecapeptide. Therefore the sequence of T1, and the sequence of the last three residues in the pentadecapeptide, is Cys-Ala-Gln. CNBr2 is also at the C-terminus of the pentadecapeptide. There is only one Arg residue, which is present in the CNBr2 and T3. It is present at the C-terminus of the T3 fragment. Overlapping CNBr2 and T3 allows the determination of the sequence. There is only one Leu residue, so T3 and CNBr1 must overlap. Overlapping CNBr1 and T3 allows the full determination of T3 (Leu-Pro-Met-Asp-Gly-Arg). Continued overlapping of the known residues allows the determination of the pentadecapeptide.

Major Spinka DVM · Answer

Are you in Agarwal's Biochem class? this question might as well be in Chinese, I have no idea how to do it lol.But you might wanna post this under Science & Mathematics rather than Business & Finance.

Prof. Cristobal Welch

The following data were obtained from partial cleavage and analysis of a pentadecapeptide:?

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