Arrange the events for the hydrolysis of amide bonds by chymotrypsin in their correct order. a. attack by ser 195 to give a tetrahedral
Please arrange the events involved in the hydrolysis of amide bonds by chymotrypsin in their correct order:
a. Attack by Ser 195, resulting in the formation of a tetrahedral intermediate.
b. Protonation by His 57 and release of the free C-terminus of the hydrolyzed bond.
c. Protonation by His 57 and release of the free N-terminus of the hydrolyzed bond.
d. Attack by water, leading to the formation of a second tetrahedral intermediate.
e. Binding of the substrate to properly position the scissile bond for cleavage.
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