Suppose your professor handed you a test tube with 2.0mL of an E. coli broth in it and told you to make a 10 t?

Question

Suppose your professor handed you a test tube containing 2.0 mL of an E. coli broth and instructed you to prepare a 10^-1 dilution of the entire culture. Please explain the steps you would take to achieve this dilution and provide the necessary calculations to support your explanation.

Anonymous · Accepted Answer

A 10^(-1) dilution means to dilute 1 in 10 [or equally, 1:9 (means 1 to 9 or 1 plus 9)].

That is, take 1 part of broth and add 9 parts of diluent.

Another way of saying, is take 1 part of broth and make it up to 10 parts with diluent.

This is the same as taking 1 mL of broth and adding 9 mL of diluent.

For the whole culture, use the 2.0 mL of broth and add 18.0 mL of diluent.

Because you are dealing with the ratio, 1:9, you can multiply both or divide both,

by the same number, and thereby end up with the same ratio.

Anonymous · Answer

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Anonymous

Suppose your professor handed you a test tube with 2.0mL of an E. coli broth in it and told you to make a 10 t?

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