Suppose your professor handed you a test tube with 2.0mL of an E. coli broth in it and told you to make a 10 t?

Question

Suppose your professor handed you a test tube with 2.0mL of an E. coli broth in it and told you to make a 10 to the negative 1 dilution of the entire culture. explain how you would do this and show calculations.

Dr. Karlee Tillman · Accepted Answer

A 10^(-1) dilution means to dilute 1 in 10 [or equally, 1:9 (means 1 to 9 or 1 plus 9)].That is, take 1 part of broth and add 9 parts of diluent.Another way of saying, is take 1 part of broth and make it up to 10 parts with diluent. This is the same as taking 1 mL of broth and adding 9 mL of diluent.For the whole culture, use the 2.0 mL of broth and add 18.0 mL of diluent.Because you are dealing with the ratio, 1:9, you can multiply both or divide both,by the same number, and thereby end up with the same ratio.

Dr. Karlee Tillman · Answer

*Eye roll* they don't seem to be some extraordinary test "grown" in a attempt tube, you do incredibly would desire to have an ova and sperm, you may't basically make a toddler out of skinny air. They do have "genuine" mothers and dads, no remember if or not the egg and sperm belong to the couple who convey them up. Now that this is cleared up, i don't see how all of us can see it as incorrect. it incredibly is a superb element that folk who will possibly not have been waiting to conceive of course can accomplish that with a sprint help. it incredibly is extraordinarily old shaped to think of that if mothers and dads can't conceive of course then they don't seem to be meant to have toddlers.

Francesco Paucek

Suppose your professor handed you a test tube with 2.0mL of an E. coli broth in it and told you to make a 10 t?

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