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Anonymous

Feb 10, 2025

In blue-white screening, what do blue colonies represent?

In the context of blue-white screening, what do blue colonies indicate? I understand that the correct answer is that they represent cells containing empty plasmid vectors. Could you please provide more insight into the significance of these blue colonies and their role in the screening process?

3 Answers

M
Myrtle Kuhn

Dec 24, 2024

The correct option is : cells containing empty plasmid vectorsExplanation:The blue-white screen is a technique which involves the rapid identification of the recombinant bacteria in a vector-based molecular cloning experiment. In this method, a DNA ligated vector is inserted in a host cell which is viable for transformation and grown in presence of X-gal.    The cells that are transformed with the vectors having the recombinant DNA produce the white colonies. Whereas, the cells transformed with empty vector i.e. the non-recombinant plasmids, produce the blue colonies.
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Anonymous

Jan 11, 2025

the burning of fossil fuels releases carbon dioxide and other greenhouse gases.good luck! =)cutie~
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Anonymous

Nov 27, 2024

b. to discover aspects of the natural world.hopefully i .

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